Principles and Practice of Chromatography

Free online book Principles and Practice of Chromatography by Raymond P. W. Scott. Chromatography, although primarily a separation technique, is mostly employed in chemical analysis. Nevertheless, to a limited extent, it is also used for preparative purposes, particularly for the isolation of relatively small amounts of materials that have comparatively high intrinsic value.


Chromatography is probably the most powerful and versatile technique available to the modern analyst. In a single step process it can separate a mixture into its individual components and simultaneously provide an quantitative estimate of each constituent. Samples may be gaseous, liquid or solid in nature and can range in complexity from a simple blend of two entantiomers to a multi component mixture containing widely differing chemical species. Furthermore, the analysis can be carried out, at one extreme, on a very costly and complex instrument, and at the other, on a simple, inexpensive thin layer plate.

Table of Contents

  • Introduction
  • The Development Process
  • Chromatography Nomenclature
  • Factors Controlling Retention
  • Factors Affecting the Magnitude of the Distribution Coefficient (K)
  • Dispersion Forces
  • Polar Forces
  • Dipole-Dipole Interactions
  • Dipole-Induced-Dipole Interactions
  • Ionic Forces
  • Molecular Forces and Chromatographic Selectivity
  • The Control of Chromatographically Available Stationary Phase (Vs)
  • Peak Dispersion in a Chromatographic Column
  • The Basic Column Chromatograph
  • The Detector Output
  • Thin Layer Chromatography Apparatus
  • Chromatography Applications
  • Liquid Chromatography Applications

Book Details

Author(s): Raymond P. W. Scott
Format(s): HTML
Link: Read online.

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